Title:	Nucleic acids db, the receptor for leptin
Document Type and Number:	United States Patent 7063958
Link to this Page:	http://www.freepatentsonline.com/7063958.html
Abstract:	The present invention relates to identification of a receptor for a satiety factor, which is involved in body weight homeostasis. Mutations in this receptor are associated with obese phenotypes. In particular, the present invention relates to identification and characterization of the receptor for leptin, including a naturally occurring soluble form of the receptor that is expected to modulate leptin activity, in particular to agonize leptin activity. The invention further relates to the nucleic acids encoding the receptor, and to methods for using the receptor, e.g., to identify leptin analogs, therapeutically, such as in gene therapy or in soluble form as an agonist or antagonist of leptin activity, or diagnostically.
Inventors:	Friedman, Jeffrey M.; Lee, Gwo-Hwa; Proenca, Ricardo;
Application Number:	599974
Filing Date:	1996-02-14
Publication Date:	2006-06-20
View Patent Images:	View PDF Images
Related Patents:	View patents that cite this patent
Export Citation:	Click for automatic bibliography generation
Assignee:	The Rockefeller University (New York, NY)
Current Classes:	435 / 69.1 , 435 / 252.31, 435 / 252.33, 435 / 252.34, 435 / 252.35, 435 / 254.21, 435 / 254.22, 435 / 255.4, 435 / 255.6, 435 / 320.1, 435 / 348, 435 / 354, 435 / 357, 435 / 358, 435 / 365, 530 / 350, 536 / 23.5
International Classes:	C12N 15/12 (20060101); C07H 21/04 (20060101)
Field of Search:	536/23.5 530/350 435/320.1,69.1,325,252.3
US Patent References:	5194596 March 1993 Tischer et al. 5350836 September 1994 Kopchick et al. 5359046 October 1994 Capon et al. 5643748 July 1997 Snodgrass et al. 6506877 January 2003 Tartaglia et al.
Foreign Patent References:	90/14092 Nov., 1990 WO 91/06666 May., 1991 WO 91/09955 Jul., 1991 WO 9712037 Sep., 1996 WO 9635787 Nov., 1996 WO 9725424 Jan., 1997 WO 9731015 Feb., 1997 WO 9712037 Apr., 1997 WO 9740380 Apr., 1997 WO 9741217 Apr., 1997 WO 9741263 Apr., 1997 WO 9742340 May., 1997 WO 9719952 Jun., 1997 WO 9725425 Jul., 1997 WO 9726523 Jul., 1997 WO
Other References:	Cioffi et al, Nature Medicine 2(5) 1996, 585-89. cited by examiner . Bennett et al, Current Biology 6(9) 1996, p. 1170-80. cited by examiner . Chen et al Cell 84 Feb. 1996, p. 491-95. cited by examiner . Chua et al, Science 271, Feb. 1996, p. 994-96. cited by examiner . Tartaglia et al Cell 83, Dec. 1995, p. 1268-71. cited by examiner . Vukicevic et al., 1996, PNAS USA 93:9021-9026. cited by examiner . Wang et al. (Nuc. Acids Res. 27: 4609-4618, 1999; p. 4617). cited by exami- ner . Kaufman et al (Blood 94: 3178-3184, 1999, pp. 3178-3184). cited by examine- r . Wigley et al. Reprod Fert Dev 6: 585-588, 1994. cited by examiner . Campbell et al. Theriology 47(1): 63-72, 1997. cited by examiner . Phillips, A., J Pharm Pharmacology 53: 1169-1174, 2001. cited by examiner . Bahary et al. (1990) Proc. Natl. Acad. Sci. USA 87:8642-6. cited by other . Bahary et al., Mammalian Genome, 4:511-515 (1993). cited by other . Bennett et al., Current Biology, 6(9): 1170-80, (1996). cited by other . Bray et al., Am. J. Clin. Nutr., 50:891-902 (1989). cited by other . Burkhardt et al. (1994) Mol. Cell. Biol., 14:1095. cited by other . Campfield et al., Science, 269:546 (1995). cited by other . Chen et al., Cell, 84: 491-95, (1996). cited by other . Chua et al., Science, 271: 994-6 (1996). cited by other . Church et al, Nature Genetics, 6:98 (1994). cited by other . Coleman, Diabetologia, 9:294-298 (1973). cited by other . Coleman et al., Diabetologia, 14:141-148 (1978). cited by other . Considine et al., J. Clin. Invest., 95:2986 (1995). cited by other . Dalton et al. (1993) Science 259:1736-9. cited by other . Davis et al. (1993) Science 260:1805-7. cited by other . Friedman et al., Genomics, 11:1054-1062 (1991). cited by other . Fukunaga, et al., EMBO, 10:2855 (1991). cited by other . Halaas et al., Science, 269:543 (1995). cited by other . Hamilton et al., Nature Med., 1:953 (1995). cited by other . Kishimoto et al. (1994) Cell 76:253-62. cited by other . Leiter, Endocrinology, 124:912-922 (1989). cited by other . Lonnqvist et al., Nature Med., 1:950 (1995). cited by other . Maffei et al., Nature Med., 1:1155 (1995). cited by other . Milatovich et al., Somatic Cell Mol. Gen., 20:75 (1994). cited by other . Modl et al., Cytogenetics Cell Genetics, 67:232 (1995). cited by other . Moll et al., Am. J. Hum. Genet., 49:1243-1255 (1991). cited by other . Morgan et al., Nucl. Acids Res., 20:5173 (1992). cited by other . Murakami et al., Proc. Natl. Acad. Sci. USA, 88:11349 (1991). cited by oth- er . Pellymounter et al., Science, 269:540, (1995). cited by other . Riley et al., Nucl. Acids Res., 18:2887 (1990). cited by other . Sanchez et al., J. Exp. Med., 178:1049 (1993). cited by other . Stephens et al., Nature, 377:530 (1995). cited by other . Tartaglia et al. (1995) Cell 83:1263-71. cited by other . Truett et al., Proc. Natl. Acad. Sci. USA, 88:7806-7809 (1991). cited by other . Turton et al., Nature, 379:69 (1996). cited by other . Zhang et al., Nature, 372:425 (1994). cited by other.
Primary Examiner:	O'Hara; Eileen B.
Attorney, Agent or Firm:	Klauber & Jackson LLC
Parent Case Data:	CROSS REFERENCE TO RELATED APPLICATION The present application is a continuation-in-part of co-pending application Ser. No. 08/586,594 filed Jan. 16, 1996. Applicants claim the benefit of this application under 35 U.S.C. .sctn. 120

Claims:

What is claimed is: 1. An isolated nucleic acid encoding a soluble leptin receptor polypeptide which is OB-Re (SEQ ID NO:10). 2. An isolated DNA molecule encoding on expression a soluble leptin receptor polypeptide selected from the group consisting of: a. a DNA molecule of SEQ ID NO: 9; and b. a DNA molecule that codes on expression for the soluble leptin receptor polypeptide encoded by the DNA molecule of subpart (a). 3. The DNA molecule of claim 2, which codes on expression for a polypeptide selected from the group consisting of: a) a leptin receptor selected from the group consisting of OB-Re (SEQ ID NO:10); and b) a leptin receptor having amino acids 28 805 of SEQ ID NO:10. 4. An isolated nucleic acid molecule having a nucleotide sequence corresponding or complementary to the DNA sequence set forth in SEQ ID NO: 9. 5. An isolated nucleic acid encoding a soluble leptin receptor selected from the group consisting of a) OB-Re (SEQ ID NO: 10); and b) a leptin receptor having amino acids 28 805 of SEQ ID NO:10. 6. The nucleic acid of claim 1, 2 or 5 which is DNA. 7. A vector comprising the DNA of claim 6. 8. An expression vector which comprises the DNA of claim 6, operatively associated with an expression control sequence. 9. A unicellular host transformed or transfected with an expression vector of claim 8. 10. The unicellular host of claim 9 selected from the group consisting of bacteria, yeast, mammalian cells, plant cells, and insect cells. 11. The unicellular host of claim 10, wherein the unicellular host is selected from the group consisting of E. coli, Pseudomonas, Bacillus, Streptomyces, Saccharomyces, Pichia, Candida, Hansenula, Torulopsis, CHO, R1.1, B-W, LM, COS 1, COS 7, BSC1, BSC40, BMT10, and Sf9 cells. 12. A method for preparing a leptin receptor polypeptide comprising: a) culturing a cell according to claim 10 under conditions that provide for expression of the leptin receptor polypeptide; and b) recovering the expressed polypeptide. 13. A unicellular host transformed or transfected with a DNA molecule of claim 6. 14. A vector comprising the DNA of claim 2, 3, or 4. 15. An expression vector which comprises the DNA of claim 2, 3, or 4, operatively associated with an expression control sequence. 16. A unicellular host transformed or transfected with an expression vector of claim 15. 17. The unicellular host of claim 16 selected from the group consisting of bacteria, yeast, mammalian cells, plant cells, and insect cells. 18. The unicellular host of claim 17, wherein the unicellular host is selected from the group consisting of E. coli, Pseudomonas, Bacillus, Streptomyces, Saccharomyces, Pichia, Candida, Hansenula, Torulopsis, CHO, R1.1, B-W, LM, COS 1, COS 7, BSC1, BSC40, BMT10, and Sf9 cells. 19. A method for preparing a leptin receptor polypeptide comprising: a) culturing a cell according to claim 17 under conditions that provide for expression of the leptin receptor polypeptide; and b) recovering the expressed polypeptide. 20. A unicellular host transformed or transfected with a DNA molecule of claim 2, 3, or 4.

Description: