Title:	Selection methods
Document Type and Number:	United States Patent 7067245
Link to this Page:	http://www.freepatentsonline.com/7067245.html
Abstract:	A rational method for obtaining a novel molecule capable of a desired interaction with a substrate of interest comprising selecting hosts or replicators which encode said novel molecules based upon cell or replicator growth caused by the desired interaction of the novel molecule and a selection molecule expressed by said host.
Inventors:	Wohlstadter, Jacob Nathaniel;
Application Number:	876343
Filing Date:	2004-06-23
Publication Date:	2006-06-27
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Assignee:
Current Classes:	435 / 4 , 435 / 235.1, 435 / 440
International Classes:	C12Q 1/00 (20060101); C12N 15/00 (20060101); C12N 7/00 (20060101)
Other References:	Yin et al. Evolution of Bacteriophage T7 in a Growing Plaque. J. Bacteriology vol. 175, pp. 1272-1277 (1993). cited by examiner.
Primary Examiner:	Brusca; John S.
Attorney, Agent or Firm:	Kramer Levin Naftalis Frankel LLP Evans, Esq.; Barry
Parent Case Data:	CROSS-REFERENCE TO RELATED APPLICATIONS This application is a continuation of U.S. application Ser. No. 09/573,830, filed May 18, 2000 now U.S. Pat. No. 6,846,628, which is a continuation of U.S. application Ser. No. 08/235,437, filed Apr. 29, 1994 now U.S. Pat. No. 6,087,177, the disclosures of which are incorporated herein by reference.

Claims:

I claim 1. A method for selecting a virus expressing a molecule having an activity of interest comprising: a) growing a population of viruses in a high selection pressure strain of bacteria where said activity of interest confers a growth advantage; b) expanding the virus population produced in step (a) by growing said population in a low selection pressure strain of bacteria; c) cycling the virus population produced in step (b) through steps (a) and (b) one or more times. 2. The method of claim 1 further comprising exposing said population of viruses to mutagenizing conditions. 3. The method of claim 2, wherein exposure to said mutagenizing conditions occurs prior to growth in said high selection pressure strain. 4. The method of claim 2, wherein exposure to said mutagenizing conditions occurs after growth in said high selection pressure strain. 5. The method of claim 1, wherein the virus is a lytic virus. 6. The method of claim 1, wherein periodic addition of bacteria occurs during step a. 7. The method of claim 1, wherein periodic addition of bacteria occurs during step b. 8. The method of claim 1, wherein periodic addition of bacteria occurs during steps a and b.

Description: