Title:	Isolated nematode regulated gene promoter and use thereof
Document Type and Number:	United States Patent 7078589
Link to this Page:	http://www.freepatentsonline.com/7078589.html
Abstract:	The present invention relates to nematode-regulated promoter and polypeptide-encoding nucleotide sequences and their use in creating or enhancing nematode-resistance in plants. Nucleic acid molecules comprising a nematode-resistance sequence operably linked to a nematode-regulated promoter and vectors, plant cells, plants, and transformed seeds containing such constructs are provided. Methods for the use of such constructs in repressing or inducing expression of a nematode-resistance sequence in a plant are also provided. In addition, methods are provided for conferring or improving nematode resistance in plants by repression or induction of nematode-resistance sequences.
Inventors:	Hu, Xu; Lu, Guihua;
Application Number:	837104
Filing Date:	2004-04-30
Publication Date:	2006-07-18
View Patent Images:	View PDF Images
Related Patents:	View patents that cite this patent
Export Citation:	Click for automatic bibliography generation
Assignee:	Pioneer Hi-Bred International, Inc. (Johnston, IA)
Current Classes:	800 / 287 , 435 / 320.1, 435 / 468, 435 / 69.1, 536 / 24.1, 800 / 278, 800 / 298, 800 / 312
International Classes:	C12N 15/09 (20060101); A01H 5/00 (20060101); C12N 15/82 (20060101)
Field of Search:	800/287,278,298,295,312,279 435/468,430,320.1,69.1 536/24.1
US Patent References:	5750386 May 1998 Conkling et al. 5994627 November 1999 Lagudah 6006470 December 1999 Geoghegan et al. 6228992 May 2001 Jessen et al. 6252138 June 2001 Karimi et al. 6448471 September 2002 Puzio et al. 2003 / 0177536 September 2003 Grundler et al.
Foreign Patent References:	WO 92/21757 Dec., 1992 WO WO 99/26483 Jun., 1999 WO
Other References:	Kim et al. Plant Molecular Biology, vol. 24, pp. 105-117, (1994). cited by examiner . Keller et al. The Plant Cell, vol. 3, pp. 1051-1061, (1991). cited by exam- iner . Urwin, et al., Continual Green-Fluorescent Protein Monitoring of Cauliflower Mosaic Virus 35S Promoter Activity in Nematode-Induced Feeding Cells in Arabidopsis thaliana, MPMI, (1997), 10(3): 394-400. cite- d by other . Puzio, et al., Isolation of regulatory DNA regions related to differentiation of nematode feeding structures in Arabidopsis thaliana, Physiol and Mol Plant Pathol, (1998), 53: 177-193. cited by other . Puzio, et al., Isolation of a gene from Arabidopsis thaliana related to nematode feeding structures, NCBI, (1999), Accession Y11994. cited by oth- er . Puzio, et al., Isolation of a gene from Arabidopsis thaliana related to nematode feeding structures GENE, (1999), 239: 163-172. cited by other . Puzio, et al., Isolation of regulatory DNA regions related to differentiation of nematode feeding structures in Arabidopsis thaliana, (2000), NCBI, Accession AJ249204. cited by other . Puzio, et al., Promoter analysis of pyk20, a gene from Arabidopsis thaliana, Plant Sci, (2000), 157:245-255. cited by other . Puzio, et al., Plasmodiophora brassicae-induced expression of pyk20, an Arabidopsis thaliana gene with glutamine-rich domain, Physiol and Mol Plant Pathol, (2000), 56: 79-84. cited by other.
Primary Examiner:	Ibrahim; Medina A.
Attorney, Agent or Firm:	Pioneer Hi-Bred International, Inc.
Parent Case Data:	CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to and benefit of U.S. Provisional Application No. 60/467,623, filed May 2, 2003.

Claims:

What is claimed is: 1. A nucleic acid molecule comprising a heterologous nematode-resistance sequence operably linked to a promoter that drives expression of said heterologous nematode-resistance sequence in a plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO:3; and (b) the nucleotide sequence deposited as Patent Deposit No. PTA-4028. 2. A vector comprising the nucleic acid molecule of claim 1. 3. A plant cell having stably incorporated into its genome the nucleic acid molecule of claim 1. 4. The plant cell of claim 3, wherein said plant cell is from a dicot plant. 5. The plant cell of claim 4, wherein said dicot plant is soybean. 6. A method of modulating the expression of a nucleotide sequence of interest in a plant cell, said method comprising stably incorporating in the genome of a plant cell said nucleotide sequence of interest operably linked to a promoter, wherein said promoter is selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO:3; and (b) the nucleotide sequence deposited as Patent Deposit No. PTA-4028. 7. A nucleic acid molecule comprising a first nucleotide sequence comprising a heterologous nematode-resistance sequence operably linked to a first promoter that induces transcription of said heterologous nematode-resistance sequence in a plant cell and a second nucleotide sequence comprising a sequence that inhibits said heterologous nematode-resistance sequence operably linked to a second promoter capable of repressing transcription of said inhibitor of said heterologous nematode-resistance sequence in a plant cell, wherein said first promoter comprises a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO:3; and (b) the nucleotide sequence deposited as Patent Deposit No. PTA-4028. 8. An expression cassette comprising the nucleic acid molecule of claim 7. 9. A plant cell having stably incorporated in its genome the nucleic acid molecule of claim 7. 10. The plant cell of claim 9, wherein said plant cell is from a dicot plant. 11. The plant cell of claim 10, wherein said dicot plant is soybean. 12. A plant stably transformed with a nucleic acid molecule comprising a heterologous nematode-resistance sequence operably linked to a promoter that induces transcription of said nematode-resistance sequence in a plant cell in response to a nematode stimulus, wherein said promoter comprises a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO:3; and (b) the nucleotide sequence deposited as Patent Deposit No. PTA-4028. 13. The plant of claim 12, wherein said plant is a dicot. 14. The plant of claim 13, wherein said dicot is soybean. 15. Transformed seed of the plant of claim 12, wherein the seed comprise the nucleic acid molecule. 16. A method for conferring or improving nematode resistance in a plant, said method comprising transforming said plant with a nucleic acid molecule comprising a heterologous sequence operably linked to a promoter that induces transcription of said heterologous sequence in a plant cell in response to a nematode stimulus and regenerating a stably transformed plant, wherein said promoter comprises a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO:3; and (b) the nucleotide sequence deposited as Patent Deposit No. PTA-4028. 17. The method of claim 16, wherein said plant is a dicot. 18. The method of claim 17, wherein said dicot is soybean. 19. A nucleic acid construct comprising a promoter, where said promoter regulates transcription of an operably linked encoding nucleotide sequence, wherein the promoter comprises a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO: 3; and (b) the nucleotide sequence deposited as patent Deposit No. PTA-4028. 20. An expression vector comprising the nucleic acid construct of claim 19. 21. A nucleic acid molecule comprising a heterologous nematode-resistance sequence operably linked to a promoter that drives expression of said heterologous nematode-resistance sequence in a plant cell, wherein said promoter comprises the nucleotide sequence set forth in SEQ ID NO:3.

Description: